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NICO NeuroWebinar: 3D-imaging after clearing: theory and applications

Published: Tuesday, March 23, 2021 by Giovanna Gambarotta

NeuroWebinar

Friday 26/3/21 at 2.00 pm - Lecture
Paolo Giacobini
Univ. Lille, Inserm, CHU Lille, Laboratory of Development and Plasticity of the Postnatal Brain, Lille Neuroscience & Cognition

3D-imaging after clearing: theory and applications

Traditional histological examination of brain systems and networks has long relied on tissue sectioning and analyses of thin serial slices that presents limitations for large volumetric imaging. In the past few years several groups have developed protocols to render tissues “optically transparent” thereby minimising light scatter and allowing inspection of neural networks within intact specimens usually lost in 2D optics.Recently developed tissue clearing methods made it possible to explore intact organs coupling immunohistochemistry in toto withlight-sheet microscopy.

In this talk theory and applications of different tissue-clearing techniques and volume imaging will be presented in the context of Neuroscience to explain how these techniques can be adapted to study the development and organization of the brain as well as of other peripheral organs. Our 3D data demonstrate that with thorough biochemical optimization, we can now detect morphogenetic processes, cell migration and terminal differentiation during embryonic and postnatal development in different species, including humans. These approaches open a novel route for high-resolution studies of brain architecture in mammals in physiological and pathological conditions.

Host: Silvia De Marchis
webex link  | Meeting ID: 121 743 8656 | Password: BVpa3dGmF42

Last update: 23/03/2021 22:09
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