NICO-Webinar: "Cell type-specific omics of neurons and glia" 27/1/23 @ 14.00
Hybrid seminar: both in presence (max 25 people in Seminar room) and on webex
Friday 27/1/23 h. 2:00 pm
Indrek Koppel,
Tallinn University of Technology, Estonia
Cell type-specific omics of neurons and glia
For studying transcriptomes in a cell type-specific manner researchers can choose between single-cell strategies and affinity purification of ribosome-associated mRNA. Single cell proteomics is in very early stages of development, but cell-specific labeling and affinity purification of proteins has been achieved using genetic tools, ensuring incorporation of affinity tags in cell types of interest. In this talk, I will discuss currently available tools in cell type-specific proteomics and introduce a method that leverages a puromycin inactivating enzyme for achieving cell type specificity. I will describe the use of this strategy for studying protein synthesis in co-cultures of rat cortex cells. In addition, I will talk about our efforts in studying neuron-astrocyte communication using tools for cell-specific activation and translatomics.
Host: Letizia Marvaldi | webex link
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