METABOLIC BIOCHEMISTRY

METABOLIC BIOCHEMISTRY

Academic year 2022/2023

Sommario del corso

• Course objectives
• Results of learning outcomes
• Course delivery
• Learning assessment methods
• Support activities
• Program
• Suggested textbooks and readings
• Teaching tools

Course objectives

This teaching contributes to the learning objectives included into the Biomolecular area of the Master in Cellular and Molecular Biology - Biologia Cellulare e Molecolare, providing knowledge and applicative abilities on organ-specific and advanced biochemistry by studying the biosynthesis and degradation of specific molecules and cofactors with high impact on metabolic balance, medicine and pharmacology. Furthermore, it aims to elucidate the molecular mechanisms underlining metabolic and hormonal regulation. In the last part, specific metabolic pathways from different organisms will be studied to understand how they are exploited for biotechnological applications.

Results of learning outcomes

KNOWLEDGE AND UNDERSTANDING.

The students will be able to:

- master advanced biochemical concepts;

- understand the concept of metabolic regulation:

- understand how hormones regulate metabolism;

- understand how xenobiotics are metabolised by organism;

- learn how some metabolic pathways can be exploited for biotechnological applications.

JUDGEMENT AUTONOMY

At the end of the teaching the students will be able to:

- understand a regulation pathway in the picture of the overall metabolism;

- exploit metabolic versatility for different purposes.

COMMUNICATION SKILL

Students will develop the ability to:

- understand conference talks from native speakers about topics descrobed during the lectures. 

Course delivery

Lectures: 48 hours, that include 2 hours of an extra-activity that is a test on the study and comprehension of two scientific reviews on a specific topic of the course.

All lessons will be delivered in presence. Alternative online teaching (by streaming) may be introduced according to the University recommendations related to the status of the COVID-19 pandemic.

Learning assessment methods

Exams will take place exclusively in presence with the only exception of students who self-declare, in relation to Covid-19, personal fragility or positivity.

Remote examinations may be introduced according to the University recommendations related to the status of the COVID-19 pandemic. 

The final exam consists of a written work with two open questions with limited time (12-15 minutes for each question) and space perfomed on Moodle platform. A third question where formulas are requested is written with 20 minutes of time.

For each question, the maximal grade will be 33. If the average will be more than 30, the exam will be marked 30 cum laude.

In any case, students should gain a sufficient grade (18 out of 30 or more) on all the three questions in order to pass the exam.

Support activities

Support activities are organized on the Moodle e-learning platform (http://biologia.i-learn.unito.it/ ).

In particular, some recent reviews about topics related to lectures will be provided.

A quiz for the assessment of the reading and comprehension is performed as an extra activity.

A forum on Moodle will be also available for any request from the students.

Program

Part 1. Advanced metabolic biochemistry.

1-    Tissue - specific metabolic regulation.

2-    Iron metabolism.

3-    Amino acid metabolism: biosynthesis of amino acids.

4-    Metabolism molecule derived from amino acids : porphyrins and heme.

5-    Protein metabolism; sorting protein destiny, chaperones and folding, protein degradation, diseases linked to proteostasis.

6-    Metabolism of xenobiotics: Phase I and Phase II

Part 2. Mtabolic and hormonal regulation

1-    Hormones: classification, structure and function. Hormone receptors, signal transduction and metabolic effects.

2-    The hormonal regulation

3-    Steroid and steroid hormones : biosynthesis and regulation . Role and regulation in the endocrine and neuroendocrine .

Part 3. Applied biochemistry

1-    Microbial metabolism and fermentation: potential applications

2-    Production of biofuels

3-    Detoxification of pollutants

David L. Nelson, Albert L. Lehninger, Lehninger principles of biochemistry.

Robert K. Murray, David A. Bender, Kathleen M. Botham, Peter J. Kennelly, Victor W. Rodwell, P. Anthony Weil. Harper's Illustrated Biochemistry 

Slides and notes of the lectures. 

Recent reviews and reserach papers indicated during the lectures and available on the Moodle e-learning platform (http://biologia.i-learn.unito.it/ ).

Class schedule

• Teaching materials
• Exams and finals
• Go to Moodle
• Visit the forums