Academic year 2022/2023
- Course ID
- Prof. Silvia De Marchis (Coordinator)
- Degree course
- Cellular and Molecular Biology
- 1st year
- Teaching period
- Semester 2
- Course disciplinary sector (SSD)
- BIO/06 - comparative anatomy and cytology
- Lessons optional and laboratories mandatory
- Type of examination
- Written and interview (optional)
Sommario del corso
This teaching contributes to the learning objectives included into the Neurobiological curriculum of the Master in Cellular and Molecular Biology - Biologia Cellulare e Molecolare, providing knowledge and applicative abilities.
The main objective is to guide students to understand the genesis of the complexity of the nervous system, by developing research skills and critical readings of scientific papers dealing with different aspects of neural development. Through the analysis of the most recent literature based on different animal and in vitro models, the students will acquire knowledge on the principles and cellular/molecular mechanisms underlying normal development of the nervous system, as well as the neurobiological bases of neurodevelopmental disorders.
Results of learning outcomes
KNOWLEDGE AND UNDERSTANDING
- identify fundamental concepts in developmental biology
- use appropriate terminology in developmental biology
- explain the principles of neural induction (molecular mechanisms and conservation among metazoa)
- associate specific genes to acquisition of regional identity in the developing nervous system
- define what is an organizing center in developing brain and provide examples
- describe the cellular/molecular mechanisms underlying neural circuit development
- identify the features and potential of neural stem/progenitors cells and define their regulation by cell-autonomous vs non autonomous factors.
- link specific cellular/molecular dysfunctions to neurodevelopmental disorders
APPLYING KNOWLEDGE AND UNDERSTANDING
- identify the best model systems to address specific scientific questions in developmental neuroscience
- grasp the core concept of a scientific paper
- find and elaborate data related to gene expression/distribution in the developing nervous system starting from public availabe resources (i.e. Allen Brain Atlas)
- find possible experimental approaches and choose the right technology to address specific questions in the field of developmental neuroscence
- write a research project
- interpretation and discussion of scientific data.
- peer revision of other students tasks
- presentation of scientific papers and/or active participation to scientific discussion following lectures and seminars.
- presentation of a research project.
- writing of short reports on scientific papers.
- learning skills will be fostered through activities with peers working in group
This year, a module of the course will be given by the Visiting Professor Paolo Giacobini (Inserm U1172), an internationally recognized specialist in the field of neural development, and expert in the development of the GnRH system in rodents and humans.
Introduction and basic knowledge in developmental biology; animal models, in vitro models and technologies for the study of nervous system development; neural induction, derivation of the nervous system in different metazoa, origin, role and molecular nature of the neural inducer; neural patterning, Hox genes and antero-posterior axis, dorso-ventral polarity of the neural tube; Cell proliferation and migration in the developing nervous tissue; Neural cell specification, determination and differentiation; Axon growth and guidance; Cerebral cortex development; Adult neurogenesis; Development of GnRH neurons, the full repertoire of molecular cues regulating their migratory process and the precise targeting of these cells to the hypothalamus. Particular emphasis will be given to new emerging technologies that can be applied synergistically to study any cell-tissue system development, such as single cell profiling, spatial transcriptomics, 2D mass cytometry, cyclic immunofluorescence and 3D imaging of optically cleared organs.
During the course, the students will be weekly guided to understand specific topics related to the main questions and established knowledge in the field of neurodevelopmental research through in classroom lectures and seminars, interactive group work, discussion time. Readings of scientific articles and online activities on moodle platform will be assigned to the students and monitored by the teacher.
All lessons will be delivered in presence. Alternative online teaching (by streaming) may be introduced according to the University recommendations related to the status of the COVID-19 pandemic.
Learning assessment methods
Tasks and activities proposed during the course will be evaluated and will points (or fractions of points) to be added to the final grade.
The final exam will be on the moodle platform: it will consist in 10 closed questions and 2 open questions based on topics covered in lectures, assigned readings, and online activities, followed by an oral examination (optional).
The final learning assessment will be in written form (Moodle platform) with optional oral integration.
Exams will take place exclusively in presence with the only exception of students who self-declare, in relation to Covid-19, personal fragility or positivity.
Remote examinations may be introduced according to the University recommendations related to the status of the COVID-19 pandemic.
IMPORTANT: Early registration to the e-learning platform (moodle) is mandatory !
Suggested readings and bibliography
There is no specific textbook for this course. For basic and general reference, see Development of the Nervous System (D.H. Sanes, T.A. Reh, W. A. Harris) Academic Press - Elsevier, 4th Ed.
Specific scientific Articles and Reviews will be uploaded on the Moodle course website.
Websites containing support videos, texts, images and other materials are also indicated.
Lessons: dal 05/03/2019 to 14/06/2019
Notes: See the timetable at the Class Schedule Page